SRA, Flow Sorting

Position Summary:

Quotient is seeking a Flow Cytometry and Fluorescence Activated Cell Sorting Senior Research Associate to join our High Throughput Platform team. The primary focus of this role is the isolation and sorting of cells and nuclei from human tissues to support downstream somatic genomics workflows, including DNA extraction and sequencing library preparation to support detection of disease relevant somatic variants. The successful candidate will work closely with our Flow Cytometry and Automation platforms to refine our processes and scale our Flow Cytometry operations. In addition, this position will provide growth opportunities to learn and contribute to disease projects across our histology and/or molecular workflows.

The ideal candidate will thrive in a fast-paced, cross-functional environment, supporting delivery across prioritized workstreams with agility, positivity, and a proactive mindset. They will demonstrate initiative in solving problems, raising concerns, and driving improvements, while remaining committed to continuous learning, open feedback, and working beyond their comfort zone.

Responsibilities:

• Conduct cell sorting experiments to isolate specific cell/nuclei populations, with high purity and viability.
• Operate and troubleshoot flow cytometry and cell sorting equipment
• Support the design and optimization of tissue dissociation and cell/nuclei isolation methods to meet project goals
• Perform the preparation and dissociation of human tissues and cells
• Perform nucleic acid extraction and sequencing library preparation.
• Work collaboratively with other team members across various functional areas.
• Maintain compliance with the Human Tissue Act (HTA) and COSHH regulations

Qualifications:

Essential

• BSc or MSc degree in a biologically relevant field
• 3+ years' experience of flow cytometry (optimizing staining conditions, Improving signal/noise, panel optimization, reagent evaluation).
• Working knowledge of the principles of cell sorting
• An understanding of the maintenance, set up and troubleshooting of flow cytometry cell sorting equipment
• Proficiency in flow cytometry data analysis software and automated analysis pipelines
• Ability to identify opportunities for process improvement and laboratory automation within flow cytometry workflows
• Use of digital laboratory information management systems (Benchling preferred)
• Strong communication and collaborative skills with the ability to work effectively in a fast-paced startup environment

Desirable

• Experience working within a flow cytometry and cell sorting facility
• Experience in isolation and sorting of nuclei
• Experience with optimization of tissue dissociation methods
• Experience working with multi-colour antibody panels for flow cytometry
• Experience and understanding of spectral flow cytometry, including unmixing and autofluorescence extraction
• Experience working with automation and understanding of how similar systems can be applied to cell dissociation and flow cytometry sorting workflows
• Experience with next generation sequencing library preparation and other downstream molecular biology processing
• Experience working with digital productivity tools such as Notion, Trello or Jira
• Experience using Benchling to create schemas, templates and automated workflows
• Knowledge and experience of working under HTA regulations
• Previous experience in a biotech or pharmaceutical industry setting

Values and Behaviors:

• Encourage respectful disagreement and cultivate open-minded, ego-free interactions to continuously push each other towards excellence
• Seek out diverse perspectives; practice active listening and genuine curiosity to ensure all contributions are valued, regardless of source
• Recognize the impact of your behavior, language and attitudes, and strive for balanced, meaningful exchanges that enhance mutual growth and understanding in all interactions
• Use a company-first mindset to guide decision-making; prioritize team over individual success
• Take calculated risks and challenge convention in the quest for exceptional outcomes

About Quotient:

Quotient Therapeutics is a privately-held, early stage company developing breakthrough medicines informed by natural somatic genetic diversity present in patients. Through our work in somatic genomics, we are forging a new status quo for biopharma research and development across a broad pipeline of internal and partnered programs.

The company was founded by Flagship Pioneering, an innovative enterprise that conceives, creates, resources, and grows first-in-category life sciences companies. Flagship Pioneering has created over 100 groundbreaking companies over the past twenty years, all of which are pioneering novel and proprietary biological, industrial, and engineering approaches to solve major needs in human health and sustainability. These companies include Moderna (MRNA), Generate Biomedicines, Sana Biotechnology (SANA), Tessera Therapeutics, Evelo Biosciences (EVLO), Indigo Agriculture, Seres Therapeutics (MCRB), and Syros Pharmaceuticals (SYRS).

Quotient Therapeutics and Flagship Pioneering are committed to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status.

Recruitment & Staffing Agencies:

Quotient Therapeutics (“Quotient”) and Flagship Pioneering (“FSP”) do not accept unsolicited resumes from any source other than candidates. The submission of unsolicited resumes by recruitment or staffing agencies to Quotient, FSP or their employees is strictly prohibited unless contacted directly by Flagship Pioneering’s internal Talent Acquisition team. Any resume submitted by an agency in the absence of a signed agreement will automatically become the property of FSP, and FSP will not owe any referral or other fees with respect thereto.