Research Technician Level II

Company Description

UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital. With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre. As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine. UHN is a research hospital affiliated with the University of Toronto. 

UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality. 

www.uhn.ca 

Job Description

Union: Non-Union
Number of Vacancies: 1
Site: Toronto General Site, Max Bell Research Building
Department: Toronto General Research Institute
Reports to: Principal Investigator
Hours: 35 per week
Shifts: Day
Status: Temporary Full-Time
Closing Date: September 10, 2025

Position Summary

The Research Technician II will be responsible for production, expression and optimization of e. Coli expressed proteins. This includes molecular biology (PCR, cloning, etc), protein expression and purification. Assays using these proteins, including enzyme kinetic assays, using fluorescent plate reader.

In addition, processing clinical samples (blood) for clinical trials and then enzyme kinetic assays. Data analyses, interpretation and preparation of graphs, tables and reports will also be required.

Duties:

• Transport samples to PI's research lab, and to other collaborators within UHN.
• Molecular biology to optimize protein expression
• Protein expression
• Protein purification
• Protein characterization
• Enzyme kinetics
• Protease assays on clinical samples (blood/plasma) or sourced plasma
• Analyze and interpret data including statistical analyses, in collaboration with biostatistician
• Assist team members with other aspects of the clinical studies

Qualifications

• BSc in Cell Biology or equivalent; MSc preferred
• Minimum 3 years related experience
• Experience isolating plasma from peripheral blood
• Intermediate molecular biology skills, including PCR, ELISA, and blotting
• Experience in Protein purification and enzyme Kinetics
• Excellent documentation skills
• Self-motivated with strong organizational skills
• Experience with statistical software and packages

Additional Information

Why join UHN?
In addition to working alongside some of the most talented and inspiring healthcare professionals in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN.

• Competitive offer packages
• Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/)
• Close access to Transit and UHN shuttle service
• A flexible work environment
• Opportunities for development and promotions within a large organization
• Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.

All applications must be submitted before the posting close date.

UHN uses email to communicate with selected candidates.  Please ensure you check your email regularly.

Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading, inaccurate or incorrect, UHN reserves the right to discontinue with the consideration of their application.

UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.

We thank all applicants for their interest, however, only those selected for further consideration will be contacted.